ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN
Streptavidin
CAS: 9013-20-1
Molecular Formula: C14H17BrClNO2S
ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN - Names and Identifiers
ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN - Physico-chemical Properties
| Molecular Formula | C14H17BrClNO2S |
| Molar Mass | 378.71228 |
| Water Solubility | Gives a clear solution at 5mg/ml in 0.1M NaCl. is soluble in water at 1 mg/mL, or in phosphate buffered saline (PBS). |
| Solubility | H2O: 0.1mg/mL, clear, colorless |
| Appearance | lyophilized powder |
| Color | White |
| Storage Condition | -20°C |
| MDL | MFCD00082035 |
| Use | This product has been widely used in the preparation of SA-coupled enzyme preparations (such as SA-HRP,SA-AP, etc.),SA-coupled fluorescein (I. e. fluorescent dyes, such as SA-FITC,SA-Cy2,SA-Cy3, etc.),SA-coupled magnetic beads, etc, furthermore, it participates in the biotechnology fields of enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, affinity chromatography packing, protein purification containing StrepTagII tags (8-AA oligopeptides), biosensors, bio-nano microspheres, pre-targeted pharmaceutical research, biochip materials, etc. |
ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN - Risk and Safety
| Risk Codes | R22 - Harmful if swallowed R36/37/38 - Irritating to eyes, respiratory system and skin. R21/22 - Harmful in contact with skin and if swallowed. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37 - Wear suitable protective clothing and gloves. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 3-10 |
| HS Code | 35040090 |
ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN - Reference
| Reference Show more | 1. Wan Tao, Xiong Wei, Jie, Wang Yuntao. Expression and clinical significance of RLIP76 and CD44 in serum and tissues of patients with gastric cancer [J]. Journal of Translational Medicine 2020 v.9;No.47(02):41-45. 2. Yu Wang, Xudong Zhao, Man Zhang, Xuan Sun, Jialei Bai, Yuan Peng, Shuang Li, Dianpeng Han, Shuye Ren, Jiang Wang, Tie, Yifei Gao, Baoning, zhixian Gao, A fluorescent amplification strategy for high-sensitive detection of 17 β-estradiol based on EXP 3. Qingkui Fang, Limin Wang, Qi Cheng, Jia Cai, Yulong Wang, Mingming Yang, Xiude Hua, Fengquan Liu, A bare-eye based one-step signal amplified semiquantitative immunochromatographic assay for the detection of imidacloprid in Chinese cabbage samples, Analytic 4. [IF=5.977] Qingkui Fang et al."A bare-eye based one-step signal amplified semiquantitative immunochromatographic assay for the detection of imidacloprid in Chinese cabbage samples."Anal Chim Acta. 2015 Jun;881:82 5. [IF=7.46] Li Hou et al."Magneto-controlled fluorescent immunosensor for sensitive determination of biomarker via three-dimensional AuNCs/liposome networks."Sensor Actuat B- Chem. 2021 Sep;342:130075 6. [IF=6.558] Yu Wang et al."A fluorescent amplification strategy for high-sensitive detection of 17 β-estradiol based on EXPAR and HCR."Anal Chim Acta. 2020 Jun;1116:1 7. [IF=4.616] Jingzhi Li et al."A highly sensitive immunofluorescence sensor based on bicolor upconversion and magnetic separation for simultaneous detection of fumonisin B1 and zearalenone."Analyst. 2021 May;146(10):3328-3335 8. [IF=2.896] Min Wei et al."Development of an electrochemical aptasensor using Au octahedra and graphene for signal amplification."Anal Methods-Uk. 2020 Jan;12(3):317-323 9. [IF=7.514] Xiaodong Cao et al."An ultrasensitive biosensor for virulence ompA gene of Cronobacter sakazakii based on boron doped carbon quantum dots-AuNPs nanozyme and exonuclease III-assisted target-recycling strategy."FOOD CHEMISTRY. 2022 Oct;391:133268 10. [IF=6.558] Yingkai Qin et al."A highly sensitive fluorometric biosensor for Fumonisin B1 detection based on upconversion nanoparticles-graphene oxide and catalytic hairpin assembly."Anal Chim Acta. 2022 May;1207:339811 11. [IF=10.618] Mei Li et al."A visual peroxidase mimicking aptasensor based on Pt nanoparticles-loaded on iron metal organic gel for fumonisin B1 analysis in corn meal."Biosens Bioelectron. 2022 Aug;209:114241 Note: For some products, our company can only provide some information. We do not guarantee the authority of the information provided, and only provide customers with reference, exchange and research. purpose: Applications for enzyme-Labelled (HRP, AP) strepta Vidin: atmosphere immunohistochemistry (EIA) storage conditions: -20 ℃ sensitivity: sensitive to heat, easy to absorb moisture Appearance: lyophilized powder 6, add an appropriate amount of preservation solution (the amount of the preservation solution can be determined according to needs, to adjust the coupling ligand magnetic beads to add an appropriate amount of preservation solution (the amount of the preservation solution can be determined according to needs, to adjust the concentration of the coupling ligand magnetic beads) stored at 4 °, if the immobilized biological ligand is stable, 0.02%(W/V) sodium azide can be added to the preservation solution as a bacteriostatic agent. 5. After the reaction is finished, the centrifuge tube is placed on a magnetic force frame, and the supernatant is removed after solid-liquid separation, then 100 μl of purified water is added and gently vortexed for 30s, and the magnetic suction separation is repeated once; 4. Add 1ml ethanolamine solution (3m, pH 9.0) in the centrifugal tube, vortex 30s, the centrifugal tube is placed in the vertical mixing instrument at room temperature reaction 1-2H; 3, after the reaction is over, the centrifugal tube is placed on the magnetic separation frame, magnetic separation to remove the supernatant, 1ml ethanolamine solution (3m, pH 9.0) was added to wash the magnetic beads twice; 2, vertical mixing reaction at room temperature for 2H, or vertical mixing at 4 °c overnight; 1. Place the centrifuge tube on the magnetic, separate and remove the supernatant, add 100 μl protein solution (25 mM MES,pH 5.0) and mix gently; (Protein concentration at 0.1-2.0mg/ml) 2.2 protein coupling 2. Add 5.0 μl MES solution (25mm, pH) into the centrifuge tube, placed on the magnetic separation, the supernatant was removed after solid-liquid separation. Repeat this step once; **3. Add the freshly prepared 50 μL EDC solution and 50 μL NHS solution (both 50mg/ml, prepared with the MES described above) to the centrifuge tube filled with magnetic beads, vortex mixing to make the magnetic beads fully suspended, vertical mixing at room temperature for 30min;** 4, after the reaction is finished, add 100 μl of the above MES solution to wash for 2 times; (activated state should not be stored for a long time, it is recommended to perform coupling immediately) 1. After fully mixing the magnetic beads, take 100μl PangoBeads COOH magnetic beads into a 1.5 centrifuge tube, place them on the magnetic separation, and remove the supernatant after solid-liquid separation; 2.1 NHS activation Ⅱ. SA coupling magnetic beads If the coated plate does not enter the downstream experiment immediately, it should be dried and stored. Before coating, 20% sucrose was added to the coating solution as an active protective agent. 4. Subsequent processes of blocking, washing, antigen-antibody binding 3. Washing: pour all the liquid in the plate hole, add 200ul of washing liquid, and let it stand for three minutes, then repeat it three times, finally, the reaction plate is inverted on the absorbent paper, so that the washing liquid in the hole is drained, the 2, and the 100ul/hole is sucked by the pipette, and the 4 ° C overnight or the 37 ° C is coated for 2H; Note: Since the isoelectric point of SA is 7.4, it is not recommended to use neutral buffer solution for coating 1. Dissolve the lyophilized powder with sodium carbonate buffer solution (pH 9.6), dilute the concentration to 3-10ug/ml (customer can set the gradient for the experiment) I. Microplate coating [brief description of Operation steps] 3. If it is used for ELISA plate, colloidal gold Gold Baking, nitrocellulose membrane with SA scribe need to be dried and stored, the coating solution or buffer solution need to be added 20% sucrose as activity protective agent. 2. Lyophilized powder containing 20mm Na2CO3 pH9.5 buffer system, if used for coating, enzyme-labeled can be used directly. If used for colloidal gold, it is best to adjust the pH or dialysis after use. Dissolution immediately after the use of the best results, not 4 ℃ long-term storage. 1. Freeze-thaw powder should avoid repeated freeze-thaw after dissolution. It is recommended to pack them into small packages and store them separately. Note |
ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN - Introduction
Streptavidin (SA) is a small molecule protein expressed by Streptomyces averidin (Streptomyces avidinii). This product is the best core structure of 127AA, each molecule SA combines 4 biotin molecules, and the international specific activity is 12 ~ 15U/mg. SA is more specific than avian avidin (AV), with a half-life of up to 6h after binding to biotin, while AV has a half-life of only 1h. SA has a lower non-specific background than AV in detection applications because SA is sugar-free and the isoelectric point is close to neutral.
Last Update：2022-10-16 17:29:57
ULTRALINK(TM) IMMOBILIZED STREPTAVIDIN - Reference Information
| Introduction | streptavidin is a 60kDa protein purified from the bacterium Streptomyces avidin. The streptavidin Homo-tetramer has a very high affinity for biotin (I. E., vitamin B7). |
| properties | the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature, its dissociation constant (Kd) is approximately 10 − 14 mol/L. Because the streptavidin-biotin complex has good tolerance to organic solvents, denaturants (such as guanidine hydrochloride), detergents (such as SDS and Triton), proteolytic enzymes, and extreme temperatures and pH, therefore, streptavidin is widely used in molecular biology and biological nanotechnology. |
| Application | in the structure, streptavidin contains the optimal core structure of SA 127AA, in the form of a homologous tetramer, four moles of biotin molecule can be bound per mole of tetramer molecule, with an international standard specific activity of 12-15 IU/mg. This property combined with biotin coupled antigen/antibody detection technology makes it widely used in the amplification of ELISA detection signal in immunology. In addition, this product can be mainly used for immune microplate, immune chromatography nitrocellulose membrane, gene chip, protein chip surface coating, rapid immobilization of biotinylated antigen, antibody, nucleic acid and other substances. Can also be used for coupling NHS magnetic beads, resin microspheres, as a luminescence diagnosis, biotinylated substance separation, magnetic test paper immunochromatography and other applications. |
Last Update：2024-04-09 21:54:55
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Product Name: Streptavidin Visit Supplier Webpage Request for quotationCAS: 9013-20-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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